Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. FOR HTRF CERTIFIED READER For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. It accepts all types of microtitration plate. a one- plate assay protocol. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER. Catalog No. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. For more information about HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Seal the plate and incubate 3 hours at RT . Specialized filters allow for IR fluorescence. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. The optical systems use two scanningHighly compatible plates for HTRF. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. Revvity. The dual measurement. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Make sure the setup for Tb Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set -up recommendations, please visit. For more. . At Cisbio we provide microplates that help you discover better. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. It can also be further streamlined to a one-. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. Reader. FOR HTRF CERTIFIED READER. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Dedicated UV-laser excitation. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Incubate 2 h at RT. Discover our high performance reader. The kit includes reagents, buffers and a microplate. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. EX: HTRF Emission range. 96-well or 384-well small volume (SV) detection. For more information about HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 5 μL of Binding buffer 2X. The detection antibody is incubated for 1. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The Negative control is used to check the non-specific signal. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Preconfigured HTRF. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. Customer Transformation Hub. Filters or monochromators for emission wavelength. As shown in Fig. Plate 25 μL of cells in. HTRF Setup. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. Quantify up to 10 analytes per well. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. The kit includes everything you need: reagents, buffers and a microplate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate readers are only certified if they. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. culture plate. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. + 4 µL HTRF ® pre-mixed antibodies . You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. Plate Reader Requirement. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF ®• HTRF®-Certified Reader. , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. Incubate overnight at RT. To perform the assay, use white plate only. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit . 5. FOR HTRF CERTIFIED READER. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. 96-well culture plateHTRF certified . FOR HTRF CERTIFIED READER For more information about HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. FOR HTRF CERTIFIED READER. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For more information about HTRF. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. For more information about HTRF. Envision Nexus. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. If not, our scientific team will help you choose the instrument that best suits your needs and budget. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Assay detection requires a microplate reader with. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. For more information about HTRF. Even if not listed, some readers may be compatible with HTRF technology. Handle complex biological matrices easily. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 2. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. Download Setup. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. • HTRF®-Certified Reader. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Read & analyse. The VICTOR Nivo is a HTRF certified microplate reader. Revvity. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. Figure 1: Principle of HTRF Cortisol competitive assay. FOR HTRF CERTIFIED READER For more information about HTRF. FOR HTRF CERTIFIED READER. The plate was covered and incubated for one hour at room temperature. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. For a list of HTRF-compatible readers and set-up recommendations, please visit . For more information about microplate recommendations, please visit our website • Store the kit at -16°C. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. HTRF Microplate Readers. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. * For HTRF microplate recommendations, please visit . White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. $352. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information. • HTRF®-Certified Reader. It features a modular design that allows users to add or change detection cartridges according to their needs. These plates can be read on all HTRF certified readers, thus making their implementation in. NC1656382. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. FOR HTRF CERTIFIED READER For. HTRF plate reader. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. The assay was prepared in both white and black plates and incubated >18 hours. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Not for use in diagnostic procedures. 8 µL cells. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. These plates can be read on all HTRF certified readers, thus making their implementation in. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. com HTRF Microplate Readers. For more information about HTRF ®Seal the plate and incubate for Overnight. FOR HTRF CERTIFIED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The ratio for each well must be calculated individually. + 4 µL HTRF pre-mixed antibodies. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. FOR HTRF CERTIFIED READER. Its. Read & analyse. No washing steps are required. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. FOR HTRF CERTIFIED READER. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. HTRF certified . 96-well culture plateFOR HTRF CERTIFIED READER. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. 5 EXAMPLE OF MAP PLATE . Dual-Luciferase® (DLR™) gene assays. Read & analyse. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Catalog No. + 4 µL HTRF pre-mixed antibodies . As shown in Fig. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. FOR HTRF CERTIFIED READER. + 4 µL HTRF ® pre-mixed antibodies . Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Step 1 Step 2 Step 3 Step 4 Step 5. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. The plate was covered both incubated for one hour at room temperature. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. For more information. 47 seconds for a 1536-well plate. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. The VICTOR Nivo is a HTRF certified microplate reader. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. As shown in Fig. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. 25,000 human Jurkat cells were plated in 96 well plates. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Make sure the setup for Eu Cryptate is used. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Make sure the setup for Eu3+ Cryptate is used. Other technologies such as imaging. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. If not, our scientific team will help you choose the instrument that best suits your needs and budget. and Canaa -8-963-4567. HTRF certified . The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. There are multiple factors to consider including: detection type, additional. Read & analyse. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. Revvity. Its. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. At Cisbio we provide microplates that help you discover better. VICTOR® Nivo™ multimode plate reader 2 | For research use only. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Your lab might even have one already. 3 and higher include optimized reader settings, as well as data analysis, for faster results. PHERAstar FSX Powerful and most sensitive HTS plate reader . Read & analyse. Your lab might even have one already. FOR HTRF CERTIFIED READER. Incubate 4h at room temperature. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. At that stage, the analyte is sandwiched between the 2 antibodies. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Figure 3. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Some vendors hold the entire market share for not only a. Incubate overnight at RT. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Plate 25 μL of cells in. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. High Speed Monochromator offers a typical wavelength accuracy of <0. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. It continues to deliver optimal results. This protocol gives the cells viability and confluence to be monitored. Incubate overnight at RT. The laser beam passes through the sample well. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Its. Specialized filters allow for IR fluorescence. Introduction. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF®-Certified Reader. Incubate 4h at RT. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. PerkinElmer. HTRF is compatible with a large range of available microplate readers. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Incubate overnight at RT. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. The ratio between control lysate signal / non-specific signal should be greater than 2. + 4 µL HTRF pre-mixed antibodies. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . Customer Transformation Hub. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Make sure the setup for Tb Cryptate is used. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 9. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. HTRF-specific. Incubate 4h at RT. Conclusion. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. 10 µL Standard or Sample. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. • HTRF®-Certified Reader **. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The plate is set up for reading and images are acquired according to specified parameters. It can also be further streamlined to a one-. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Configure your microplate reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. (665 nm and 620 nm) on a compatible HTRF ® reader. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 14 seconds for a 384-well plate. FOR HTRF CERTIFIED READER. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. As shown in Fig. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Incubate 4h at RT.